[1]黄柳,张瑞宁,田小超,等.内皮祖细胞与冠心病患者CD14CD16+单核细胞共培养后移植心肌梗死大鼠对血管密度及心肌梗死面积的影响[J].心血管病学进展,2020,(2):203-207.[doi:10.16806/j.cnki.issn.1004-3934.2020.02.027]
　HUANG Liu,ZHANG Ruining,TIAN Xiaochao,et al.Effects of Co-cultured Endothelial Progenitor Cells and CD14++CD16+ Monocytes from Coronary Heart Disease Patients on Vascular Density and Myocardial Infarction Size in Transplanting Myocardial Infarction Rats[J].Advances in Cardiovascular Diseases,2020,(2):203-207.[doi:10.16806/j.cnki.issn.1004-3934.2020.02.027]
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内皮祖细胞与冠心病患者CD14CD16+单核细胞共培养后移植心肌梗死大鼠对血管密度及心肌梗死面积的影响()
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《心血管病学进展》[ISSN:51-1187/R/CN:1004-3934]

卷:
期数:
2020年2期
页码:
203-207
栏目:
论著
出版日期:
2020-02-25

文章信息/Info

Title:
Effects of Co-cultured Endothelial Progenitor Cells and CD14++CD16+ Monocytes from Coronary Heart Disease Patients on Vascular Density and Myocardial Infarction Size in Transplanting Myocardial Infarction Rats
作者:
黄柳; 张瑞宁; 田小超; 郭炳彦; 刘素云; 李拥军
河北医科大学第二医院心血管内科，河北 石家庄 050000
Author(s):
HUANG Liu;  ZHANG Ruining;  TIAN Xiaochao;  GUO Bingyan;  LIU Suyun;  LI Yongjun
(Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, China)
关键词:
冠心病; 心肌梗死; CD14CD16+单核细胞; 内皮祖细胞
Keywords:
Coronary heart disease;  Myocardial infarction;  CD14CD16+ monocyte;  Endothelial progenitor cell
DOI:
10.16806/j.cnki.issn.1004-3934.2020.02.027
摘要:
目的 研究内皮祖细胞（endothelial progenitor cell，EPC）与冠心病患者CD14CD16+单核细胞共培养后移植心肌梗死大鼠对血管密度及心肌梗死面积的影响。方法 比较冠心病患者及健康志愿者外周血中CD14CD16+单核细胞水平，将冠心病患者外周血中分选出的CD14CD16+单核细胞与健康志愿者的EPC体外共培养观察其对EPC的增殖、迁移、黏附的影响后，将CD14CD16+单核细胞与EPC共培养的细胞移植进入心肌梗死大鼠模型，观察其对大鼠缺血心肌新生血管密度、坏死心肌面积及心功能指标的影响。结果 冠心病患者外周血中CD14CD16+单核细胞水平显著高于健康志愿者（P<0.05）；冠心病患者外周血中CD14CD16+单核细胞与健康志愿者的EPC体外共培养后增殖能力、黏附细胞数、迁移细胞数均显著降低（P<0.05）；与假手术组大鼠相比，移植4周后模型组、EPC组、CD14CD16+共培养组左心室梗死面积均显著升高（P<0.05），且EPC组<模型组（p><0.05），新生毛细管密度、左心室射血分数、左心室短轴缩短率均显著降低（p><0.05），且epc组 />CD14CD16+共培养组>模型组（P<0.05）。结论 冠心病患者外周血中CD14CD16+单核细胞水平升高，并抑制EPC修复及逆转左室重构改善心肌功能的生理作用。
Abstract:
Objective To study effects of co-cultured endothelial progenitor cells (EPC) and CD14CD16+ monocytes of coronary heart disease (CHD) patients on vascular density and myocardial infarction size intransplanting myocardial infarction rats . Methods The level of CD14++CD16+ monocytes in peripheral blood was compared between CHD patients and healthy volunteers. The in vitro co-culture was performed on CD14++CD16+ monocytes in peripheral blood of CHD patients and EPC of healthy volunteers,the influences which on proliferation, migration and adhesion of EPC cells were observed. The co-cultured cells of CD14++CD16+ monocytes and EPC were transplanted into myocardial infarction rat models. The influences on neovascular density of ischemic myocardium, necrosis myocardial area and cardiac function indexes were observed. Results The level of CD14++CD16+ monocytes in peripheral blood of CHD patients was significantly higher than that of healthy volunteers (P<0.05). After in vitro co-culture withCD14++CD16+ monocytes fromCHD patients, the proliferation ability, number of adhesion cells and number of migration cells of EPC were significantly decreased (P<0.05). Compared with sham operation group, at 4 weeks after transplantation, left ventricular infarction area was significantly increased in model group, EPC group, and CD14++CD16+ co-culture group (P<0.05). Arranging the area from small to large, the corresponding order was EPC group, CD14++CD16+ co-culture group and model group (P<0.05), whilenew capillary density, left ventricular ejection fraction and left ventricular fractional shortening were significantly decreased (P<0.05) in these three groups,whichwere of the highest value in EPC group, followed by CD14++CD16+ co-culture group and model group (P<0.05). Conclusion The level of CD14++CD16+ monocytes in peripheral blood of CHD patients is increased, which can inhibit EPC repairing, reversing of left ventricular remodeling, and improving physiological role of myocardial function

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金青年科学基金项目（81400217）国家自然科学基金面上项目（81570345）河北省医学科学研究重点课题计划（20190501）
更新日期/Last Update: 2020-04-14