[1]杨帆 吴建军.五味子乙素通过半胱天冬酶凋亡途径对抗高糖诱导的心肌细胞氧化应激损伤[J].心血管病学进展,2022,(2):188.[doi:10.16806/j.cnki.issn.1004-3934.2022.02.022]
　YANG Fan,WU Jianjun.Sch.B Protects High Glucose-Induced Cardiomyocytes from Oxidative Stress Injury via Caspase Pathway[J].Advances in Cardiovascular Diseases,2022,(2):188.[doi:10.16806/j.cnki.issn.1004-3934.2022.02.022]
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五味子乙素通过半胱天冬酶凋亡途径对抗高糖诱导的心肌细胞氧化应激损伤()
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《心血管病学进展》[ISSN:51-1187/R/CN:1004-3934]

卷:
期数:
2022年2期
页码:
188
栏目:
论著
出版日期:
2022-02-25

文章信息/Info

Title:
Sch.B Protects High Glucose-Induced Cardiomyocytes from Oxidative Stress Injury via Caspase Pathway
作者:
杨帆1; 2 吴建军2
(1.哈尔滨医科大学附属第二医院心肌缺血教育部重点实验室,黑龙江 哈尔滨150001;2.哈尔滨医科大学附属第二医院心血管内科,黑龙江 哈尔滨 150001)
Author(s):
YANG Fan1; 2; WU Jianjun2
(1.Key Laboratory of Myocardial Ischemia,Ministry of Education,Harbin Medical University, Harbin 150001,Heilongjiang,China;2.Department of Cardiology,The Second Affiliated Hospital of Harbin Medical University,Harbin 150001,Heilongjiang,China)
关键词:
糖尿病; 五味子乙素; 氧化应激; 细胞凋亡
Keywords:
Diabetes; Schisandrin B; Oxidative stress; Apoptosis
DOI:
10.16806/j.cnki.issn.1004-3934.2022.02.022
摘要:
目的 研究五味子乙素（Sch.B）是否可通过降低活性氧（ROS）的生成保护糖尿病状态下心肌细胞；是否通过抑制细胞凋亡减轻高糖诱导的心肌细胞损伤。方法 链脲佐菌素诱导Wistar大鼠制备1型糖尿病模型。口服药物Sch.B 4周后进行取材和检测。心肌细胞分为低糖组和高糖组，高糖＋Sch.B组和高糖＋氧自由基清除剂组。DHE染色检测各组细胞ROS的生成，并用Hoechst33258检测各组细胞凋亡情况。结果 Sch.B组大鼠心肌组织排列相对规整，染色均一。Sch.B组与糖尿病组大鼠相比凋亡相关蛋白半胱天冬酶-3/9和线粒体中细胞色素C表达下降。DHE染色可发现，Sch.B处理后能明显降低高糖诱导心肌细胞的ROS生成。Hoechst33258染色可发现，Sch.B和氧自由基清除剂处理后可明显降低高糖处理心肌细胞凋亡的比例。结论 Sch.B可抑制糖尿病状态下心肌细胞胞浆和线粒体中ROS的生成，抑制心肌细胞氧化应激反应，降低线粒体途径的细胞凋亡保护心肌细胞。
Abstract:
Objective The aim of the present study was to investigate : if Sch.B could alleviate the injury of cardiomyocytes by reducing the generation of ROS; if cell apoptosis could be decreased by Sch.B. Methods STZ-induced Wistar rats were established for type 1 diabetes model and observed after oral administration of Sch.B for 4 weeks. Cardiomyocytes were divided into groups as follows:control group,high glucose(HG) group,HG+Sch.B group and HG+NAC ( N-Acetyl-L-cysteine) group. Results Our results showed that the arrangement of myocardial tissue in Sch.B group was relatively regular and the staining showed uniform. After treatment with Sch.B,the expression of apoptosis-related proteins Caspase-3/9 and cytochrome C in mitochondria decreased compared with diabetic rats. DHE staining showed that Sch.B treatment could significantly reduce the ROS production of cardiomyocytes in HG. Hoechst33258 staining showed that Sch.B and NAC treatment could significantly reduce the proportion of cell apoptosis in HG-induced group. Conclusion Sch.B can protect cardiomyocytes by reducing the production of ROS in the cytoplasm and mitochondria,and inhibit cellular oxidative stress through mitochondria apoptotic pathway

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更新日期/Last Update: 2022-08-19