[1]叶莎 杨翠玲 郑媛媛.骨髓间充质干细胞来源外泌体通过PI3K/Akt途径减轻H2O2诱导心肌细胞损伤[J].心血管病学进展,2022,(3):269-273.[doi:10.16806/j.cnki.issn.1004-3934.2022.03.000]
　YE Sha,YANG Cuiling,ZHENG Yuanyuan.Bone Marrow Mesenchymal Stem Cells Derived Exosomes Attenuate H 2O2 Induced Cardiomyocyte Injury Via PI3K/Akt Pathway[J].Advances in Cardiovascular Diseases,2022,(3):269-273.[doi:10.16806/j.cnki.issn.1004-3934.2022.03.000]
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骨髓间充质干细胞来源外泌体通过PI3K/Akt途径减轻H2O2诱导心肌细胞损伤()
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《心血管病学进展》[ISSN:51-1187/R/CN:1004-3934]

卷:
期数:
2022年3期
页码:
269-273
栏目:
论著
出版日期:
2022-03-25

文章信息/Info

Title:
Bone Marrow Mesenchymal Stem Cells Derived Exosomes Attenuate H 2O2 Induced Cardiomyocyte Injury Via PI3K/Akt Pathway
文章编号:
202105120
作者:
叶莎 杨翠玲 郑媛媛
翠玲 郑媛媛(西安交通大学第二附属医院老年心血管科,陕西 西安 710000)
Author(s):
YE Sha;  YANG Cuiling;  ZHENG Yuanyuan
(Department of Geriatric Cardiology,The Second Affiliated Hospital of Xian Jiaotong University, Xian 710000,Shaanxi,China) (2020FS-254) E-mail:yesha1981@sina.com
关键词:
骨髓间充质干细胞; 外泌体; 过氧化氢; 凋亡; PI3K/Akt途径
Keywords:
Bone marrow mesenchymal stem cell s; Exosomes; Hydrogen peroxide; Apoptosis; PI3K/Akt pathway
DOI:
10.16806/j.cnki.issn.1004-3934.2022.03.000
摘要:
目的 研究骨髓间充质干细胞（BMMSC）来源外泌体通过PI3K/Akt途径减轻过氧化氢（H2O2）诱导心肌细胞损伤。方法 培养心肌H9c2细胞，采用0.5 mmol/L H2O2诱导心肌细胞损伤，给予BMMSC来源外泌体、对照溶剂二甲基亚砜或PI3K抑制剂LY294002干预，检测细胞存活率、凋亡率及Bcl-2、Bax、cleaved caspase-3、p-PI3K和p-Akt的表达水平。结果 H 2O2组细胞的存活率及Bcl-2、p-PI3K和p-Akt的表达水平低于对照组，凋亡率以及Bax和cleaved caspase-3的表达水平高于对照组（P＜0.05）；H2O2+外泌体组细胞的存活率及Bcl-2、p-PI3K和p-Akt的表达水平高于H2O2组，凋亡率以及Bax和cleaved caspase-3的表达水平低于H2O2组（P＜0.05）；LY294002+H2O2+外泌体组细胞的存活率以及Bcl-2、p-PI3K和p-Akt的表达水平低于溶剂对照+H2O2+外泌体组，凋亡率以及Bax和cleaved caspase-3的表达水平高于溶剂对照+H2O2+外泌体组（P＜0.05）。结论 BMMSC来源外泌体通过激活PI3K/Akt途径减轻H2O2诱导心肌细胞损伤。
Abstract:
Objective To study the effect of bone marrow mesenchymal stem cells (BM MSC) derived exosomes attenuate H2O2 induced cardiomyocyte injury via PI3K/Akt pathway. Methods H9c2 cells were cultured,0.5 mmol/L H 2O2 was used to induce cardiomyocyte injury. BMMSC derived exosomes,DMSO or PI3K inhibitor LY294002 were used to intervene. The cell survival rate,apoptosis rate and the expression levels of Bcl-2,Ba x,cleaved caspase-3,p-PI3K and p-A kt were detected. Results The cell survival rate and the expression levels of Bcl-2,p-PI3K and p-A kt of H 2O2 group were lower than those of control group,while the apoptosis rate and the expression levels of Bax and cleaved caspase-3 were higher than those of control group( P<0.05). The cell survival rate and the expression levels of Bcl-2,p-PI3K and p-Akt of H 2O2+exosome group were higher than those of H2O2 group,while the apoptosis rate and the expression levels of Bax and cleaved caspase-3 were lower than those of H 2O2 group( P< 0.05). The cell survival rate and the expression levels of Bcl-2,p-PI3K and p-Akt of LY294002+H 2O2+exosomes group were lower than those of solvent control+H2O2+exosomes group,while the apoptosis rate and the expression levels of Bax and cleaved caspase-3 were higher than those of solvent control+H 2O2+exosomes group(P< 0.05). Conclusion BMMSC derived exosomes attenuate H 2O2 induced cardiomyocyte injury by activating PI3K/Akt pathway.

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更新日期/Last Update: 2022-04-20