[1]高爱红,田伟.外周血环状RNA DLGAP4与类风湿关节炎合并心力衰竭的关系[J].心血管病学进展,2021,(8):759-762.[doi:10.16806/j.cnki.issn.1004-3934.2021.08.020]
 GAO Aihong,TIAN Wei.Relationship Between Circular RNA DLGAP4 in Peripheral Blood and Rheumatoid Arthritis Complicated with Heart Failure[J].Advances in Cardiovascular Diseases,2021,(8):759-762.[doi:10.16806/j.cnki.issn.1004-3934.2021.08.020]
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外周血环状RNA DLGAP4与类风湿关节炎合并心力衰竭的关系()
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《心血管病学进展》[ISSN:51-1187/R/CN:1004-3934]

卷:
期数:
2021年8期
页码:
759-762
栏目:
论著
出版日期:
2021-08-25

文章信息/Info

Title:
Relationship Between Circular RNA DLGAP4 in Peripheral Blood and Rheumatoid Arthritis Complicated with Heart Failure
作者:
高爱红1田伟2
(1.陕西省核工业二一五医院心血管内科,陕西 咸阳7120002.陕西省核工业二一五医院病理科,陕西 咸阳712000)
Author(s):
GAO Aihong1 TIAN Wei2
(1.Department of Cardiovascular Medicine,The 215th Hospital of Shaanxi Nuclear Industry,Xianyang 712000,Shaanxi,China 2. Department of Pathology,The 215th Hospital of Shaanxi Nuclear Industry,Xianyang 712000,Shaanxi,China)
关键词:
类风湿关节炎心力衰竭环状RNADLGAP4微小RNA-143
Keywords:
Rheumatoid arthritis Heart failure Circular RNA DLGAP4 MicroRNA-143
DOI:
10.16806/j.cnki.issn.1004-3934.2021.08.020
摘要:
目的 探讨外周血环状RNA(circRNA)DLGAP4表达水平与类风湿关节炎(RA)合并心力衰竭(HF)的关系。方法 选取本院2017年8月—2019年8月期间收治的RA患者42例为研究对象,根据是否合并HF分为RA组(n=28)和RA+HF组(n=14),并选取同期进行体检的健康人30例为对照组。酶联免疫吸附试验检测外周血中肿瘤坏死因子-α(TNF-α)和白介素-6(IL-6)水平,qRT-PCR技术检测外周血中circDLGAP4和miR-143的表达水平,Spearman相关分析circDLGAP4、miR-143表达水平与RA合并HF的相关性,并利用受试者工作特征曲线(ROC)评估circDLGAP4、miR-143表达水平诊断RA合并HF的效能。结果 RA组患者外周血中TNF-α、IL-6和miR-143水平高于对照组,circDLGAP4表达水平低于对照组,RA+HF组患者的TNF-α、IL-6和miR-143水平高于RA组,circDLGAP4表达水平低于RA组,差异均有统计学意义(P<0.05);Spearman相关分析结果显示:circDLGAP4表达水平与TNF-α、IL-6和miR-143均呈负相关(r=-0.5207、-0.4327和-0.7567,P均<0.05);ROC结果显示:circDLGAP4诊断RA合并HF的AUC为0.772,95% CI为0.621~0.923,截断值为0.67时的敏感度为83.3%,特异度为73.3%。结论 RA患者外周血中circDLGAP4表达水平显著降低,且合并HF时降低更显著,同时circDLGAP4表达水平对RA合并HF的诊断有一定的价值。
Abstract:
Objective To explore the relationship between circular RNA (circRNA) DLGAP4 in peripheral blood and rheumatoid arthritis (RA) complicated with heart failure (HF). Methods 42 patients with RA admitted to our hospital from August 2017 to August 2019 were enrolled as research objects and divided into RA(n=28) and RA+HF group (n=14) based on whether complicated with HF. 30 healthy people contemporarily underwent physical examination were selected as control. Levels of TNF-α and IL-6 in peripheral blood were detected by ELISA,and expressions of circDLGAP4 and miR-143 were tested by qRT-PCR. Correlations of circDLGAP4 and miR-143 with RA complicated with HF were calculated by Spearman analysis. The diagnostic efficiency of circDLGAP4 and miR-143 for RA complicated with HF was evaluated by receiver operating characteristic (ROC) curves. Results Levels of TNF-α,IL-6 and miR-143 in peripheral blood were higher while with a lower expression of circDLGAP4 in RA group than control. Compared with RA group,levels of TNF-α,IL-6 and miR-143 were elevated accompanied with a declined expression of circDLGAP4 in RA+HF group(P<0.05). Results of Spearman analysis showed the expression of circDLGAP4 was negatively correlated with TNF-α,IL-6 and miR-143 (r=-0.5207,-0.4327,-0.7567,P<0.05). ROC curves indicated AUC and 95% CI of circDLGAP4 in diagnosis for RA complicated with HF were 0.772 and 0.621 ~0.923,respectively. When cut-off value was 0.67,the sensitivity and specificity were 83.3% and 73.3% respectively. Conclusion The expression level of circDLGAP4 in peripheral blood of RA patients is significantly decreased with a further decline when complicated with HF. Expression of circDLGAP4 would be of certain value in diagnosis for RA complicated with HF

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备注/Memo

备注/Memo:
基金项目:陕西省自然科学基金项目(2013JM4106) 通信作者:田伟,E-mail:2529021166@qq.com 收稿日期:2020-04-27
更新日期/Last Update: 2021-09-26