[1]张淼 魏冠平 黄煜 何庆.模拟急性高原缺氧对小鼠一氧化氮相关通路的影响[J].心血管病学进展,2020,(9):984-988.[doi:10.16806/j.cnki.issn.1004-3934.20.09.023]
 ZHANG Miao,WEI Guanping,HUANG Yu,et al.Effect of Simulated Acute High Altitude Hypoxia on Nitric Oxide-related Pathways in Mice[J].Advances in Cardiovascular Diseases,2020,(9):984-988.[doi:10.16806/j.cnki.issn.1004-3934.20.09.023]
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模拟急性高原缺氧对小鼠一氧化氮相关通路的影响()
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《心血管病学进展》[ISSN:51-1187/R/CN:1004-3934]

卷:
期数:
2020年9期
页码:
984-988
栏目:
出版日期:
2020-09-25

文章信息/Info

Title:
Effect of Simulated Acute High Altitude Hypoxia on Nitric Oxide-related Pathways in Mice
作者:
张淼1 魏冠平1 黄煜2 何庆12
(1.西南交通大学医学院,四川 成都 610031;2.西南交通大学附属医院 成都市第三人民医院,四川 成都 610031)
Author(s):
ZHANG Miao1WEI Guanping1HUANG Yu2;HE Qing12
(1.Medical School of Southwest Jiaotong University, Chengdu 610031SichuanChina;2.The Affiliated Hospital of Southwest Jiaotong University, The Third Peoples Hospital of Chengdu, Chengdu 610031, Sichuan, China)
关键词:
缺氧一氧化氮不对称二甲基精氨酸L-精氨酸
Keywords:
HypoxiaNitric oxideAsymmetric dimethylarginineL-arginine
DOI:
10.16806/j.cnki.issn.1004-3934.20.09.023
摘要:
目的 研究急性高原缺氧对小鼠一氧化氮(NO)相关通路的影响,并探讨补充L-精氨酸对NO含量的影响。方法 将40只小鼠随机分为4组:常氧对照组(N组)、缺氧模型组(M组)、低剂量L-精氨酸组(LL组)和高剂量L-精氨酸(HL组),每组10只。连续10 d于同一时间点按体重灌胃给药。其中,前3 d于正常环境中给药,N组和M组给予生理盐水。随后,将小鼠置于模拟海拔4 000 m的低压低氧动物实验舱中饲养1周。采用酶联免疫吸附剂测定法检测肺组织不对称二甲基精氨酸(ADMA)、内皮型一氧化氮合酶(eNOS)和Ⅰ型精氨酸酶(ARGⅠ)浓度,硝酸还原酶法检测肺组织NO含量。结果 与N组小鼠体重增加相比,M组、LL组和HL组小鼠体重减轻(P<0.01,P<0.01,P<0.05),与M组相比,LL组和HL组体重变化无统计学意义。与N组相比,M组、LL组和HL组ADMA浓度显著增加,差异均有统计学意义(P<0.01)。与M组相比,LL组和HL组ADMA浓度明显下降(P<0.01,P<0.01),其中,HL组ADMA浓度<LL组(P<0.01)。M组NO含量>N组、LL组和HL组,但差异均无统计学意义。与N组相比,M组eNOS和ARGⅠ的浓度明显增加(P<0.05,P<0.01)。M组eNOS浓度>LL组(P<0.01)。与M组相比,LL组和HL组的ARGⅠ浓度降低,LL组ARGⅠ浓度<HL组(P<0.01)。M组和HL组ARGⅠ/eNOS>LL组(P<0.01,P<0.01)。结论 急性高原缺氧会导致NO相关通路失衡,其可能机制为ADMA对eNOS的竞争性抑制作用和eNOS与ARGⅠ之间的相互竞争,补充L-精氨酸未能增加NO含量。
Abstract:
Objective To study the effect of acute high altitude hypoxia on nitric oxide-related pathway in mice, and explore the effect of L-arginine supplementation on NO level. Methods A total of 40 mice were randomly divided into four groups: normoxic control group (group N), hypoxic model group (group M), low-dose L-arginine group (group LL) and high-dose L-arginine group (group HL), with 10 mice in each group. At the same point for 10 consecutive days, intragastric administration was performed according to body weight. During the first three days, the L-arginine was administered in a normal environment, and the group N and group M were given normal saline simultaneously. Then, the mice were raised in a hypobaric hypoxic animal experimental chamber at a simulated altitude of 4000 m for one week. Concentration of lung tissue asymmetric dimethylarginine (ADMA), endothelial nitric oxide synthase (eNOS) and arginaseⅠ(ARGⅠ) were detected using enzyme-linked immunosorbent assay, and content of NO in lung tissue was detected by nitrate reductase method. Results Mice in group N gained weight, while mice in group M, group LL and group HL lost weight (P<0.01P<0.01P<0.05. Compared with group M, the weight change of group LL and HL showed no statistical significance. The ADMA concentration in group M, group LL and group HL was higher than that in group N with statistical significance (P<0.01. Compared with group M, ADMA concentration in group LL and group HL decreased significantly(P < 0.01;P<0.01), and it was lower in group HL than that in group LLP < 0.01). The content of NO in group M was higher than that in group N, group LL and group HL, but the difference was not statistically significant. Compared with group N, the concentrations of eNOS and ARG I in group M were significantly increased (P<0.05P<0.01. The concentration of eNOS in group LL decreased compared with that in group M (P<0.01. Compared with group M, ARG Ⅰ concentration in group LL and group HL reduced, and it was lower in group LL than in group HL (P < 0.01). The ratio of ARGⅠ/eNOS in group M and group HL was higher than that in group LL (P<0.01P<0.01. Conclusion Acute plateau hypoxia could lead to imbalance of NO-related pathways, which may be attributed to competitive inhibition of eNOS by ADMA and competition between eNOS and ARG Ⅰ. Supplementation of L-arginine failed to increase NO content.

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备注/Memo

备注/Memo:
收稿日期:2020-03-04 基金项目:国家自然科学基金(81501640) 通作者:何庆,E-mailkk555888@126.com
更新日期/Last Update: 2020-12-04