[1]罗芩 何庆?/html>.腺苷2B受体激动剂对大鼠高原肺水肿的治疗作用[J].心血管病学进展,2021,(10):941.[doi:10.16806/j.cnki.issn.1004-3934.2021.10.019]
　LUO Qin,HE Qing.Therapeutic Effect of Adenosine 2B Receptor Agonist on High-Altitude Pulmonary Edema in Rats[J].Advances in Cardiovascular Diseases,2021,(10):941.[doi:10.16806/j.cnki.issn.1004-3934.2021.10.019]
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腺苷2B受体激动剂对大鼠高原肺水肿的治疗作用()
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《心血管病学进展》[ISSN:51-1187/R/CN:1004-3934]

卷:
期数:
2021年10期
页码:
941
栏目:
论著
出版日期:
2021-10-25

文章信息/Info

Title:
Therapeutic Effect of Adenosine 2B Receptor Agonist on High-Altitude Pulmonary Edema in Rats
作者:
罗芩 何庆?/html>
( 1．西南交通大学医学院,四川 成都 610036;2．西南交通大学附属医院 成都市第三人民医院,四川 成都 610036)
Author(s):
LUO Qin1; HE Qing2
College of Medcine,Southwest Jiaotong University,Chengdu 610036,Sichuan,China;2. The Affiliated Hospital of Southwest Jiaotong University,The Third People’s Hospital of Chengdu,Chengdu 610036,Sichuan,China) E-mail123hq@163.com
关键词:
高原肺水肿; 腺苷2B受体; BAY60-6583; 上皮Na+通道
Keywords:
High-altitude pulmonary edema;  Adenosine 2B receptor; BAY60-6583; Epithelial sodium channel
DOI:
10.16806/j.cnki.issn.1004-3934.2021.10.019
摘要:
目的 研究腺苷2B受体激动剂BAY60-6583对高原肺水肿的治疗作用。方法 24只SD 雄性大鼠随机分为平原对照组（Control组）、高原肺水肿模型组（HAPE组）和BAY60-6583干预组（BAY组），每组8只。除Control组外， HAPE组及BAY组均以10 m/s的速度上升至模拟海拔6 000 m高度并维持48 h。出舱后BAY组腹腔注射BAY60-6583 [2 mg/(kg·d)]，Control组及HAPE组腹腔注射等体积溶媒，持续3d。大鼠麻醉后收集血液和组织标本，取肺叶HE染色观察肺组织病理变化，测定肺含水量、超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量、肿瘤坏死因子-α（TNF-α）含量、白介素（IL）-6含量、环磷酸腺苷（cAMP）含量和上皮Na+通道α亚基（ENaC-α）的基因相对表达量与蛋白含量。结果 与Control组相比，HAPE组肺组织出现明显病理学变化，肺含水量升高， TNF-α、IL-6和MDA含量升高，SOD活性和ENaC-α明显降低（P＜0.05）；与HAPE组相比，BAY组肺组织病理学变化明显改善，肺组织含水量明显降低，TNF-α、IL-6和MDA含量降低，SOD活力、cAMP和ENaC-α明显升高（P＜0.05）。结论 BAY60-6583 可能通过降低肺含水量、TNF-α、IL-6和MDA含量，提高SOD活性、cAMP水平和ENaC-α的含量，以减轻大鼠高原低氧环境所致的肺水肿。
Abstract:
Objective To study the therapeutic effect of adenosine 2B receptor agonist BAY60-6583 on high-altitude pulmonary edema. Methods 24 male SD rats were randomly divided into plain control group (Control), high-altitude pulmonary edema model group (HAPE) and BAY60-6583 intervention group (BAY), with 8 rats in each group. Except for the Control group, rats in HAPE and BAY group were raised to simulated altitude of 6 000 m at a speed of 10 m/s for 48 hours. After exiting , rats in BAY group were intraperitoneally injected with BAY60-6583 [2mg/(kg·d)],and rats in Control and HAPE groups were intraperitoneally injected with equal volume of solvent for 3 days. Blood and tissue samples were collected after anesthesia. Pulmonary pathological changes were observed by HE staining. The water content of lung tissue, SOD activity, contents of MDA,TNF-α,IL-6,cAMP,and content of epithelial Na+ channel α subunit (ENAC-α) were detected. Results Compared with Control group, HAPE group showed significant pathological changes in lung tissue, with increased contents of lung water, TNF-α,IL-6 and MDA, decreased SOD activity and ENAC-α expressions(P＜0.05). Compared with HAPE group, pulmonary histopathological changes in BAY group were significantly improved, accompanied with declined water contents of lung tissue, declined contents of TNF-α,IL-6,MDA,and elevated SOD activity, cAMP and ENAC-α contents (P＜0.05). Conclusion BAY60-6583 may alleviate pulmonary edema caused by high-altitude hypoxia environment in rats by decreasing contents of lung water, TNF-α,IL-6,MDA,and increasing SOD activity, contents of cAMP and ENAC-α.

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更新日期/Last Update: 2021-12-02