[1]王丹 张旭涛 卢艳雨 戴东普 孟心国 刘永荣 张彬彬.冬凌草甲素缓解脂多糖诱导的脓毒症心肌损伤[J].心血管病学进展,2025,(5):470.[doi:10.16806/j.cnki.issn.1004-3934.2025.05.019]
　WANG Dan,ZHANG Xutao,LU Yanyu,et al.Oridonin Alleviates Lipopolysaccharide-Induced Septic Myocardial Injury[J].Advances in Cardiovascular Diseases,2025,(5):470.[doi:10.16806/j.cnki.issn.1004-3934.2025.05.019]
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冬凌草甲素缓解脂多糖诱导的脓毒症心肌损伤()

《心血管病学进展》[ISSN:51-1187/R/CN:1004-3934]

卷:
期数:
2025年5期
页码:
470
栏目:
论著
出版日期:
2025-05-25

文章信息/Info

Title:
Oridonin Alleviates Lipopolysaccharide-Induced Septic Myocardial Injury
作者:
王丹1 张旭涛 2 卢艳雨1 戴东普1 孟心国1 刘永荣3 张彬彬1
(1.郑州大学第一附属医院心血管内科,河南 郑州 450052;2.武汉大学人民医院心血管内科,湖北 武汉 430060;3.重庆市合川区人民医院心内科,重庆市401520)
Author(s):
WANG Dan 1; ZHANG Xutao2; LU Yanyu1; DAI Dongpu1; MENG Xinguo1; LIU Yongrong3; ?HANG Binbin1?/html>
?1. Department of Cardiology,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,Henan,China; 2. Department of Cardiology,Renmin Hospital of Wuhan University, Wuhan 430060,Hubei,China; 3. Department of Cardiology,Hechuan District People’s Hospital,Chongqing 401520,China)
关键词:
冬凌草甲素; 脂多糖; 脓毒症心肌损伤; 氧化应激
Keywords:
Oridonin; Lipopolysaccharide; Sepsis-induced myocardial injury; Oxidative stress
DOI:
10.16806/j.cnki.issn.1004-3934.2025.05.019
摘要:
目的 探索冬凌草甲素(Ori)在脂多糖(LPS)诱导的脓毒症心肌损伤中的作用与机制。方法 将 6~8周C57BL/6J小鼠随机分为3组：对照组、LPS组、Ori组，每组8只小鼠，通过LPS诱导脓毒症心肌损伤。利用超声心动图检测小鼠的心功能变化。血清生化检测各组小鼠的超氧化物歧化酶(SOD)、丙二醛(MDA)、肌酸激酶同工酶(CK-MB)和乳酸脱氢酶水平。HE染色评估小鼠的心肌损伤与炎症细胞浸润情况。蛋白印迹法检测各组小鼠左心室心肌中核转录因子红系2相关因子2(Nrf2)和血红素加氧酶1(HO-1) 表达水平。结果 超声心动图显示Ori改善LPS诱导的小鼠心功能不全。血清生化检测显示，LPS导致小鼠SOD表达水平下降，MDA、LDH、CK-MB表达水平升高，而给予Ori处理的小鼠可上调SOD表达水平，下调MDA、LDH、CK-MB表达水平。蛋白印迹法检测显示Ori腹腔注射明显上调Nrf2和HO-1的表达水平。结论 Ori可有效提升心肌抗氧化应激能力，缓解LPS诱导的心肌损伤，其可能通过Nrf2/HO-1通路发挥作用。
Abstract:
Objective To explore the role and mechanism of ori donin (Ori) in septic myocardial injury induced by lipopolysaccharide (LPS). Methods 6-8 weeks C57BL/6J mice were randomly divided into 3 groups :the ontrol group ,the LPS group and the Ori group,with 8 mice in each group,and septic myocardial injury was subsequently induced by LPS administration. The changes of cardiac function in mice were detected by echocardiography. Serum biochemical detection of superoxide dismutase (SOD),malondialdehyde (MDA),creatine kinase isoenzyme (CK-MB) and lactate dehydrogenase (LDH) levels in mice in each group was performed. HE staining was used to evaluate myocardial injury and inflammatory cell infiltration in mice. The expression levels of nuclear factor erythroid 2-related factor 2/heme oxygenase 1(Nrf2/HO-1) i n left ventricular myocardium of mice in each group was detected by Western blotting. Results Echocardiography showed that Ori improved LPS-induced cardiac dysfunction in mice. Serum biochemical detection showed that SOD decreased and MDA ,LDH and CK-MB increased in mice treated with LPS,while SOD increased and MDA,LDH and CK-MB expression levels decreased in mice treated with Ori. Western blotting showed that intraperitoneal injection of Ori significantly up-regulated the expression levels of Nrf2 and HO-1. Conclusion Ori can effectively enhance myocardial anti-oxidative stress and alleviate LPS-induced myocardial injury,which may be played by Nrf2/HO-1 pathway

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更新日期/Last Update: 2025-06-26