[1]任红梅 杨阳 单世富 马丽娜 李振军 席少静.miR-155-5p对大鼠急性心肌梗死致心肌凋亡的影响研究[J].心血管病学进展,2024,(10):948.[doi:10.16806/j.cnki.issn.1004-3934.2024.10.016]
 REN Hongmei,YANG Yang,SHAN Shifu,et al.Study on the Effect of miR-155-5p on Myocardial Apoptosis Induced by Acute Myocardial Infarction in Rats[J].Advances in Cardiovascular Diseases,2024,(10):948.[doi:10.16806/j.cnki.issn.1004-3934.2024.10.016]
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miR-155-5p对大鼠急性心肌梗死致心肌凋亡的影响研究()
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《心血管病学进展》[ISSN:51-1187/R/CN:1004-3934]

卷:
期数:
2024年10期
页码:
948
栏目:
论著
出版日期:
2024-10-25

文章信息/Info

Title:
Study on the Effect of miR-155-5p on Myocardial Apoptosis Induced by Acute Myocardial Infarction in Rats
作者:
任红梅 杨阳 单世富 马丽娜 李振军 席少静
(宁夏回族自治区人民医院心内科,宁夏 银川 750001)
Author(s):
REN HongmeiYANG YangSHAN ShifuMA LinaLI ZhenjunXI Shao jing
(Cardiology Department,Peoples Hospital of Ningxia Hui Autonomous Region,Yinchuan,750001,Ningxia,China) [
关键词:
心肌梗死miR-155-5p凋亡
Keywords:
Myocardial infarction miR-155-5pApoptosis
DOI:
10.16806/j.cnki.issn.1004-3934.2024.10.016
摘要:
目的 本研究探讨了miR-155-5p在大鼠急性心肌梗死模型中的保护作用。方法 通过向健康雄性SD大鼠注射空载体和miR-155-5p腺病毒,分为空载体模型组和miR-155-5p过表达组,研究其对心脏功能、心肌损伤指标和心肌细胞凋亡的影响。结果 结果显示,miR-155-5p过表达组的心脏功能显著改善,心肌损伤标志物降低,心肌梗死面积和细胞凋亡减少。进一步,miR-155-5p过表达也能增强H9C2心肌细胞在缺氧条件下的存活能力,降低凋亡相关蛋白的表达。结论 这表明miR-155-5p的过表达可以有效保护心肌细胞,减轻急性心肌缺血引起的心肌损伤,有望成为心肌梗死治疗的新靶点。此研究为miR-155-5p在心肌梗死诊断和治疗中的应用提供了新的理论依据。
Abstract:
Objective This study investigates the protective role of miR-155-5p in a rat model of acute myocardial infarction. Methods Healthy male SD rats were injected with either a control vector or miR-155-5p adenovirus, forming control model groups and miR-155-5p overexpression groups, to study the effects on cardiac function, myocardial injury markers, and myocardial cell apoptosis. Results The results showed that the cardiac function of the miR-155-5p overexpression group significantly improved, myocardial injury markers were reduced, and the area of myocardial infarction and cell apoptosis were decreased. Additionally, overexpression of miR-155-5p also enhanced the survival ability of H9C2 cardiomyocytes under hypoxic conditions and reduced the expression of apoptosis-related proteins. Conclusion This indicates that the overexpression of miR-155-5p can effectively protect myocardial cells and alleviate myocardial damage caused by acute myocardial ischemia, potentially serving as a new target for the treatment of myocardial infarction. This study provides a new theoretical basis for the application of miR-155-5p in the diagnosis and treatment of myocardial infarction.

参考文献/References:

[1] Song P,Fang Z,Wang H,et al. Global and regional prevalence,burden,and risk factors for carotid atherosclerosis:a systematic review,meta-analysis,and modelling study[J]. Lancet Glob Health,2020,8(5):e721-e729.

[2] Roth GA,Mensah GA,Johnson CO,et al. Global burden of cardiovascular diseases and risk factors,1990-2019:update from the GBD 2019 study[J]. J Am Coll Cardiol,2020,76(25):2982-3021.

[3] Xue J,Chen L,Cheng H,et al. The identification and validation of hub genes associated with acute myocardial infarction using weighted gene co-expression network analysis[J]. J Cardiovasc Dev Dis,2022,9(1):30.

[4] Gonzalez MJ,Brito R,Gajardo A I,et al. Myocardial reperfusion injury and oxidative stress:therapeutic opportunities[J]. World J Cardiol,2018,10(9):74-86.

[5] Saad N,Duroux RI,Touitou I,et al. MicroRNAs in inflammasomopathies[J]. Immunol Lett,2023,256-257:48-54.

[6] Peng C,Li J. Editorial:MicroRNAs in endocrinology and cell signaling[J]. Front Endocrinol (Lausanne),2022,13:1118426.

[7] Zhang J. Non-coding RNAs and angiogenesis in cardiovascular diseases:a comprehensive review[J]. Mol Cell Biochem,2024. DOI:10.1007/s11010-023-04919-5.

[8] Elton TS,Selemon H,Elton SM,et al. Regulation of the MIR155 host gene in physiological and pathological processes[J]. Gene,2013,532(1):1-12.

[9] Daily ZA,Al-Ghurabei BH,Al-Qarakhli AMA,et al. MicroRNA-155 (miR-155) as an accurate biomarker of periodontal status and coronary heart disease severity:a case-control study[J]. BMC Oral Health,2023,23(1):868.

[10] Luo Y,Deng X,Chen Q,et al. Up-regulation of miR-155 protects against chronic heart failure by inhibiting HIF-1alpha[J]. Am J Transl Res,2023,15(11):6425-6436.

[11] Hu C,Liao J,Huang R,et al. MicroRNA-155-5p in serum derived-exosomes promotes ischaemia-reperfusion injury by reducing CypD ubiquitination by NEDD4[J]. ESC Heart Fail,2023,10(2):1144-1157.

[12] Xi J,Li QQ,Li BQ,et al. MiR-155 inhibition represents a potential valuable regulator in mitigating myocardial hypoxia/reoxygenation injury through targeting BAG5 and MAPK/JNK signaling[J]. Mol Med Rep,2020,21(3):1011-1020.

[13] Chen JG,Xu XM,Ji H,et al. Inhibiting miR-155 protects against myocardial ischemia/reperfusion injury via targeted regulation of HIF-1alpha in rats[J]. Iran J Basic Med Sci,2019,22(9):1050-1058.

[14] Wu HY,Liu K,Zhang JL. LINC00240/miR-155 axis regulates function of trophoblasts and M2 macrophage polarization via modulating oxidative stress-induced pyroptosis in preeclampsia[J]. Mol Med,2022,28(1):119.

[15] Wang C,Zhang C,Liu L,et al. Macrophage-derived mir-155-containing exosomes suppress fibroblast proliferation and promote fibroblast inflammation during cardiac injury[J]. Mol Ther,2017,25(1):192-204.

[16] Wang H,Bei Y,Huang P,et al. Inhibition of miR-155 protects against LPS-induced cardiac dysfunction and apoptosis in mice[J]. Mol Ther Nucleic Acids,2016,5(10):e374.

[17] Yang Y,Guo Z,Chen W,et al. M2 macrophage-derived exosomes promote angiogenesis and growth of pancreatic ductal adenocarcinoma by targeting E2F2[J]. Mol Ther,2021,29(3):1226-1238.

[18] Shi Y,Li K,Xu K,et al. MiR-155-5p accelerates cerebral ischemia-reperfusion injury via targeting DUSP14 by regulating NF-κB and MAPKs signaling pathways[J]. Eur Rev Med Pharmacol Sci,2020,24(3):1408-1419.

[19] Wang Q,Li C,Zhu Z,et al. miR-155-5p antagonizes the apoptotic effect of bufalin in triple-negative breast cancer cells[J]. Anticancer Drugs,2016,27(1):9-16.

[20] Shi Y,Li Z,Li K,et al. miR-155-5p accelerates cerebral ischemia-reperfusion inflammation injury and cell pyroptosis via DUSP14/ TXNIP/NLRP3 pathway[J]. Acta Biochim Pol,2022,69(4):787-793.

[21] Luo Y,Fu X,Ru R,et al. CpG oligodeoxynucleotides induces apoptosis of human bladder cancer cells via caspase-3-Bax/Bcl-2-p53 axis[J]. Arch Med Res,2020,51(3):233-244.

[22] Seervi M,Rani A,Sharma AK,et al. ROS mediated ER stress induces Bax-Bak dependent and independent apoptosis in response to Thioridazine[J]. Biomed Pharmacother,2018,106:200-209.

[23] Huang YK,Chang KC,Li CY,et al. AKR1B1 represses glioma cell proliferation through p38 MAPK-mediated Bcl-2/BAX/caspase-3 apoptotic signaling pathways[J]. Curr Issues Mol Biol,2023,45(4):3391-3405.

[24] Czabotar PE,Garcia-Saez AJ. Mechanisms of BCL-2 family proteins in mitochondrial apoptosis[J]. Nat Rev Mol Cell Biol,2023,24(10):732-748.

[25] Edlich F. Bcl-2 proteins and apoptosis:recent insights and unknowns[J]. Biochem Biophys Res Commun,2018,500(1):26-34.

[26] Fan L,He Z,Wang L,et al. Alterations of Bax/Bcl-2 ratio contribute to NaAsO2 induced thyrotoxicity in human thyroid follicular epithelial cells and SD rats[J]. Ecotoxicol Environ Saf,2023,264:115449.

[27] Azimian H,Dayyani M,Toossi M,et al. Bax/Bcl-2 expression ratio in prediction of response to breast cancer radiotherapy[J]. Iran J Basic Med Sci,2018,21(3):325-332.

[28] Del Principe MI,Dal Bo M,Bittolo T,et al. Clinical significance of bax/bcl-2 ratio in chronic lymphocytic leukemia[J]. Haematologica,2016,101(1):77-85.

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更新日期/Last Update: 2024-11-01