[1]王清 马学龄 梁小燕 美迪娜?叶尔肯 芦颜美.超声引导下起搏器植入构建犬慢性心力衰竭模型的方法[J].心血管病学进展,2024,(5):466.[doi:10.16806/j.cnki.issn.1004-3934.2024.05.018]
　WANG Qing,MA Xueling,LIANG Xiaoyan,et al.Ultrasound-Guided Pacemaker Implantation to Construct A Canine Model of Chronic Heart Failure[J].Advances in Cardiovascular Diseases,2024,(5):466.[doi:10.16806/j.cnki.issn.1004-3934.2024.05.018]
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超声引导下起搏器植入构建犬慢性心力衰竭模型的方法()
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《心血管病学进展》[ISSN:51-1187/R/CN:1004-3934]

卷:
期数:
2024年5期
页码:
466
栏目:
论著
出版日期:
2024-05-25

文章信息/Info

Title:
Ultrasound-Guided Pacemaker Implantation to Construct A Canine Model of Chronic Heart Failure
作者:
王清1 马学龄 2 梁小燕1 美迪娜?叶尔肯 1 芦颜美
(1.新疆医科大学第一附属医院心脏中心起搏电生理科 新疆心电生理与心脏重塑重点实验室,新疆 乌鲁木齐 830054;2.新疆医科大学第一附属医院昌吉分院超声诊断科,新疆 昌吉 831100)
Author(s):
WANG Qing1; MA Xueling2; LIANG Xiaoyan1; MeidinaYeerken1; LU Yanmei1
(1. Department of Pacing and Electrophysiology, Xinjiang Key Laboratory of Cardiac Electrophysiology and Cardiac Remodeling,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,Xinjiang,China; 2. Department of Ultrasound Diagnosis ,Changji Branch ,The First Affiliated Hospital of Xinjiang Medical University,Changji 831100,Xinjiang,China)
关键词:
超声引导; 起搏器; 犬; 慢性心力衰竭
Keywords:
Ultrasound-guided; Pacemaker; Canine; Chronic heart failure
DOI:
10.16806/j.cnki.issn.1004-3934.2024.05.018
摘要:
目的 探讨超声引导下起搏器植入构建犬慢性心力衰竭（CHF）模型的方法。方法 随机选择健康犬5只，在超声引导下，将人用起搏电极经颈外静脉植入右心室心尖部，连接人用起搏器后，将其放置右侧颈部囊袋中，采用心室非同步起搏模式，180次/min（3 d），220次/min（3 d ），250次/min（输出电压4.0 V，脉宽0.5 ms，3周）。通过观察起搏前后犬的临床表现、超声心动图、血浆脑钠肽（BNP）水平以及心脏形态学变化来评价犬CHF模型构建的成效。结果 5只犬经右心室起搏4周后均出现食欲减退、呼吸困难、活动量减少等临床表现，超声心动图证实左室射血分数降低，酶联免疫吸附试验检测血浆BNP水平增加，病理染色结果显示胶原纤维增多，透射电子显微镜结果显示心肌肌原纤维断裂、肌节损伤、Z线消失、线粒体溶解和破碎。结论 采用超声引导经颈外静脉植入人用起搏器建立的犬CHF模型操作方便，成功率高，是一种安全高效的CHF模型构建方法。
Abstract:
Objective To explore the method of constructing canine chronic heart failure(CHF) model by ultrasound-guided pacemaker implantation. Methods Five healthy canines were randomly selected. Under the guidance of ultrasound,the human pacemaker electrode was implanted into the right ventricular apex through the external jugular vein. After connecting the human pacemaker,it was placed in the right neck pocket. The ventricular asynchronous pacing mode was used with a pacing frequency of 180 bpm for 3 days,followed by 220 bpm for 3 days and finally 250 bpm for 3 weeks(output voltage 4.0 V,pulse width 0.5 ms). The effectiveness of constructing a canine CHF model was assessed by the clinical manifestations,echocardiography results,plasma brain natriuretic peptide(BNP) levels,and cardiac morphological changes before and after pacing. Results After four weeks of right ventricular pacing,five canines showed clinical manifestations such as loss of appetite,dyspnea and decreased activity. Echocardiography confirmed a decrease in left ventricular ejection fraction. The plasma BNP level was increased by ELISA. The results of pathological staining showed that collagen fibers were increased. Transmission electron microscopy results showed that myocardial myofibrils were broken,sarcomeres were damaged,Z-lines were disappeared,and mitochondria were dissolved and cracked. Conclusion The canine model of CHF established by ultrasound-guided external jugular vein implantation of a human pacemaker is easy to operate and has a high success rate. It is a safe and efficient method for constructing a CHF model.

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更新日期/Last Update: 2024-06-28