[1]文江艳 滕藤 唐其柱.黄杞苷减轻H9c2细胞的缺氧再复氧损伤[J].心血管病学进展,2024,(6):566.[doi:10.16806/j.cnki.issn.1004-3934.2024.06.020]
 WEN Jiangyan,TENG Teng,TANG Qizhu.Engeletin Alleviates Hypoxia Reoxygenation Injury in H9c2 Cells[J].Advances in Cardiovascular Diseases,2024,(6):566.[doi:10.16806/j.cnki.issn.1004-3934.2024.06.020]
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黄杞苷减轻H9c2细胞的缺氧再复氧损伤()
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《心血管病学进展》[ISSN:51-1187/R/CN:1004-3934]

卷:
期数:
2024年6期
页码:
566
栏目:
论著
出版日期:
2024-06-25

文章信息/Info

Title:
Engeletin Alleviates Hypoxia Reoxygenation Injury in H9c2 Cells
作者:
文江艳 滕藤 唐其柱
(武汉大学人民医院心血管内科 代谢与相关慢病湖北省重点实验室,湖北 武汉 430060)
Author(s):
WEN JiangyanTENG TengTANG Qizhu
(Department of Cardiology,Renmin Hospital of Wuhan University,Hubei Key Laboratory of Metabolic and Chronic Diseases,Wuhan 430060,Hubei,China)
关键词:
黄杞苷缺氧再复氧氧化应激细胞凋亡
Keywords:
Engeletin Hypoxia reoxygenation Oxidative stress Cell apoptosis
DOI:
10.16806/j.cnki.issn.1004-3934.2024.06.020
摘要:
目的 探讨黄杞苷对 H9c2细胞缺氧再复氧损伤的影响及作用机制。方法 构建 H9c2细胞缺氧再复氧模型,将H9c2细胞随机分为正常组、正常+黄杞苷组、缺氧再复氧组和缺氧再复氧+黄杞苷组。实时荧光定量聚合酶链式反应检测相关抗氧化酶(过氧化物歧化酶2、谷胱甘肽过氧化物酶1、过氧化氢酶)的mRNA水平;试剂盒检测超氧化物歧化酶、心肌损伤标志物、丙二醛以及胱天蛋白酶-3(caspase-3)水平;免疫组织化学染色检测细胞氧化应激水平;原位末端标记法染色检测细胞凋亡水平;western blot检测相关蛋白核转录因子红系2相关因子2(Nrf-2)、血红素加氧酶1(HO-1)表达水平。结果 正常组与正常+黄杞苷组心肌损伤标志物、氧化应激、细胞凋亡等指标及 Nrf-2、HO-1蛋白表达比较,无统计学差异(P>0.05);与正常组比较,缺氧再复氧组心肌损伤标志物、活性氧、丙二醛、细胞凋亡率以及caspase-3水平明显升高,相关抗氧化酶的mRNA水平、超氧化物歧化酶水平及Nrf-2、HO-1蛋白表达明显下降(P<0.05);与缺氧再复氧组比较,缺氧再复氧+黄杞苷组心肌损伤标志物、活性氧、丙二醛、细胞凋亡率以及caspase-3水平明显下降,相关抗氧化酶的mRNA水平、超氧化物歧化酶水平及Nrf-2、HO-1蛋白表达明显升高(P<0.05)。结论 黄杞苷可以减轻缺氧再复氧的 H9c2细胞的氧化应激损伤及细胞凋亡,可能是通过Nrf-2/HO-1通路发挥作用。
Abstract:
Objective To investigate the effect and mechanism of Huangqi glycoside on hypoxia reoxygenation injury in H9c2 cells. Method A hypoxia reoxygenation model of H9c2 cells was constructed ,and H9c2 cells were randomly divided into normal group ,normal+Huangqi glycoside group,hypoxia reoxygenation group,and hypoxia reoxygenation+Huangqi glycoside group. Real-time quantitative polymerase chain reaction detection of mRNA levels of related antioxidant enzymes(peroxidase 2,glutathione peroxidase 1,catalase). The kit detects levels of superoxide dismutase,myocardial injury markers,malondialdehyde,and caspase-3. Immunohistochemical staining was used to detect cellular oxidative stress levels . TdT-mediated dUTP nick end labeling staining was used to detect the level of cell apoptosis . Western blot was used to detect the expression levels of red blood cell nuclear factor 2 related factor 2( Nrf-2) and heme oxygenase 1(HO-1) related proteins.Results There was no statistically significant difference in myocardial injury markers,oxidative stress,cell apoptosis,and Nrf-2,HO-1 protein expression between the normal group and the normal+Huangqi glycoside group (P>0.05). Compared with the normal group ,the hypoxia reoxygenation group showed a significant increase in myocardial injury markers,active oxygen,malondialdehyde,cell apoptosis rate,and caspase-3 levels,while the mRNA levels of related antioxidant enzymes,superoxide dismutase levels,and Nrf-2,HO-1 protein expression decreased significantly(P<0.05). Compared with the hypoxia reoxygenation group ,the hypoxia reoxygenation+Huangqi glycoside group showed a significant decrease in myocardial injury markers,active oxygen,malondialdehyde,cell apoptosis rate,and caspase-3 levels. The mRNA levels of related antioxidant enzymes,superoxide dismutase levels,and Nrf-2,HO-1 protein expression were significantly increased(P<0.05). Conclusion Huangqi glucoside can alleviate oxidative stress damage and apoptosis in H9c2 cells subjected to hypoxia reoxygenation ,possibly through the Nrf-2/HO-1 pathway

参考文献/References:

[1] Tsao CW,Aday AW,Almarzooq ZI,et al. Heart Disease and Stroke Statistics-2023 Update:a report from the American Heart Association[J]. Circulation,2023,147(8):e93-e621.

[2] Bergmark BA,Mathenge N,Merlini PA,et al.Acute coronary syndromes[J]. Lancet,2022,399(10332):1347-1358.

[3] Lecour S,Andreadou I,B?tker HE,et al. IMproving Preclinical Assessment of Cardioprotective Therapies (IMPACT) criteria:guidelines of the EU-CARDIOPROTECTION COST Action[J]. Basic Res Cardiol,2021,116(1):52.

[4] Dong L,Shen Z,Chi H,et al. Research progress of chinese medicine in the treatment of myocardial ischemia-reperfusion injury[J]. Am J Chin Med,2023,51(1):1-17.

[5] Xiang M,Lu Y,Xin L,et al. Role of oxidative stress in reperfusion following myocardial ischemia and its treatments[J]. Oxid Med Cell Longev,2021,2021:6614009.

[6] Popov SV,Mukhomedzyanov AV,Voronkov NS,et al. Regulation of autophagy of the heart in ischemia and reperfusion[J]. Apoptosis,2023,28(1-2):55-80.

[7] Heusch G. Myocardial ischaemia-reperfusion injury and cardioprotection in perspective[J]. Nat Rev Cardiol,2020,17(12):773-789.

[8] Xu Y,Zhang J,Yu L,et al. Engeletin alleviates depression-like phenotype by iNormoxiareasing synaptic plasticity via the BDNF-TrkB-mTORC1 signalling pathway[J]. J Cell Mol Med,2023,27(23):3928-3938.

[9] Zhao X,Chen R,Shi Y,et al. Antioxidant and anti-inflammatory activities of six flavonoids from smilax glabra roxb[J]. Molecules,2020,25(22):5295.

[10] Wang H,Jiang Z,Pang Z,et al. Engeletin protects against TNF-α-induced apoptosis and reactive oxygen species generation in chondrocytes and alleviates osteoarthritis in vivo[J]. J Inflamm Res,2021,14:745-760.

[11] Wang C,La L,Feng H,et al. Aldose reductase inhibitor engeletin suppresses pelvic inflammatory disease by blocking the phospholipase C/protein kinase C-dependent/NF-κB and MAPK cascades[J]. J Agric Food Chem,2020,68(42):11747-11757.

[12] Alam F,Mohammadin K,Shafique Z,et al. Citrus flavonoids as potential therapeutic agents:a review [J]. Phytother Res,2022,36(4):1417-1441.

[13] Li B,Yang X,Zhang P,et al. Engeletin alleviates the inflammation and apoptosis in intervertebral disc degeneration via inhibiting the NF-κB and MAPK pathways[J]. J Inflamm Res,2022,15:5767-5783.

[14] Kuppe C,Ramirez Flores RO,Li Z,et al. Spatial multi-omic map of human myocardial infarction[J]. Nature,2022,608(7924):766-777.

[15] Alfonso F,Cuesta J,Diez-Villanueva P.PCI for ischemic left ventricular dysfunormoxiation[J]. N Engl J Med,2023,388(2):188.

[16] Ferdinandy P,Andreadou I,Baxter GF,et al. Interaction of cardiovascular nonmodifiable risk factors,comorbidities and comedications with ischemia/reperfusion injury and cardioprotection by pharmacological treatments and ischemic conditioning[J]. Pharmacol Rev,2023,75(1):159-216.

[17] Xiang Q,Yi X,Zhu XH,et al. Regulated cell death in myocardial ischemia-reperfusion injury[J]. Trends Endocrinol Metab,2024,35(3):219-234.

[18] Zhang G,Wang X,Li C,et al. Integrated stress response couples mitochondrial protein translation with oxidative stress control[J]. Circulation,2021,144(18):1500-1515.

[19] Sies H,Belousov VV,Chandel NS,et al. Defining roles of specific reactive oxygen species(ROS) in cell biology and physiology[J]. Nat Rev Mol Cell Biol,2022,23(7):499-515.

[20] Songbo M,Lang H,Xinyong C,et al. Oxidative stress injury in doxorubicin-induced cardiotoxicity[J]. Toxicol Lett,2019,307:41-48.

[21] Prag HA,Murphy MP,Krieg T.Preventing mitochondrial reverse electron transport as a strategy for cardioprotection[J]. Basic Res Cardiol,2023,118(1):34.

[22] Liu H,Li S,Xu Y,et al. Engeletin protects against cerebral ischemia/reperfusion injury by modulating the VEGF/vasohibin and Ang-1/Tie-2 pathways[J]. Braz J Med Biol Res,2021,54(10):e11028.

[23] Xu Y,Zhang J,Gao F,et al. Engeletin alleviates cerebral ischemia reperfusion-induced neuroinflammation via the HMGB1/TLR4/NF-κB network[J]. J Cell Mol Med,2023,27(12):1653-1663.

[24] Bock FJ,Tait SWG.Mitochondria as multifaceted regulators of cell death[J]. Nat Rev Mol Cell Biol,2020,21(2):85-100.

[25] Jiang Y,Chen X,Fan M,et al. TRAIL facilitates cytokine expression and macrophage migration during hypoxia/reoxygenation via ER stress-dependent NF-κB pathway[J]. Mol Immunol,2017,82:123-136.

[26] Zhang J,Chen X,Chen H,et al. Engeletin ameliorates pulmonary fibrosis through endoplasmic reticulum stress depending on lNormoxia949-mediated TGF-β1-Smad2/3 and JNK signalling pathways[J]. Pharm Biol,2020,58(1):1105-1114.

[27] Hejazian SM,Hosseiniyan Khatibi SM,Barzegari A,et al. Nrf-2 as a therapeutic target in acute kidney injury[J]. Life Sci,2021,264:118581.

[28] Lu X,Xu G,Lin Z,et al. Sulforaphane delays intervertebral disc degeneration by alleviating endoplasmic reticulum stress in nucleus pulposus cells via activating Nrf-2/HO-1[J]. Oxid Med Cell Longev,2023,2023:3626091.

[29] Huang Z,Ji H,Shi J,et al. Engeletin attenuates abeta1-42-induced oxidative stress and neuroinflammation by Keap1/Nrf2 pathway[J]. Inflammation,2020,43(5):1759-1771.

[30] Fang Z,Liu Z,Tao B,et al. Engeletin mediates antiarrhythmic effects in mice with isoproterenol-induced cardiac remodeling[J]. Biomed Pharmacother,2023,161:114439.

更新日期/Last Update: 2024-07-26