[1]刘江文 王凤媛 李彬 杨双 吴浩亮 唐艳红 王晞 黄从新.鞣花酸灌胃对脂多糖诱导的脓毒症心功能不全的影响[J].心血管病学进展,2023,(2):181-185.[doi:10.16806/j.cnki.issn.1004-3934.2023.02.019]
　LIU JiangwenWANG FengyuanLI BinYANG ShuangWU HaoliangTANG YanhongWANG XiHUANG Congxin.Effects of Ellagic Acid intragastric Administration on Cardiac Insufficiency Induced by Lipopolysaccharide Sepsis[J].Advances in Cardiovascular Diseases,2023,(2):181-185.[doi:10.16806/j.cnki.issn.1004-3934.2023.02.019]
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鞣花酸灌胃对脂多糖诱导的脓毒症心功能不全的影响()
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《心血管病学进展》[ISSN:51-1187/R/CN:1004-3934]

卷:
期数:
2023年2期
页码:
181-185
栏目:
论著
出版日期:
2023-02-25

文章信息/Info

Title:
Effects of Ellagic Acid intragastric Administration on Cardiac Insufficiency Induced by Lipopolysaccharide Sepsis
作者:
刘江文 王凤媛 李彬 杨双 吴浩亮 唐艳红 王晞 黄从新
(武汉大学人民医院心内科 武汉大学心血管病研究所 心血管病湖北省重点实验室,湖北 武汉 430060)
Author(s):
LIU JiangwenWANG FengyuanLI BinYANG ShuangWU HaoliangTANG YanhongWANG XiHUANG Congxin
(Department of Cardiology,Renmin Hospital of Wuhan University,Cardiovascular Research Institute of Wuhan University,Hubei Key Laboratory of Cardiology,Wuhan 430060,Hubei,China)
关键词:
鞣花酸; 脓毒症; 氧化应激
Keywords:
Ellagic acid;  Sepsis;  Oxidative stress
DOI:
10.16806/j.cnki.issn.1004-3934.2023.02.019
摘要:
目的 探究鞣花酸（EA）对脂多糖（LPS）诱导的脓毒症所致心功能不全保护作用的潜在机制。方法 将32只C57BL/6小鼠分为4组：对照Control（Con）组（n=8），LPS组（n=8），20EA（20 mg/kg）组（n=8），40EA（40 mg/kg）组（n=8）。20EA及40EA组用EA灌胃14 d 后腹腔注射10 mg/kg LPS，Sham组使用等量生理盐水灌胃。超声心动图评测心功能，分析射血分数及短轴缩短率，生化法检测超氧化物岐化酶（SOD）、丙二醛（MDA）、肌酸激酶同工酶（CK-MB）及乳酸脱氢酶（LDH）血清水平。结果 超声心动图显示EA以剂量依赖的方式改善LPS诱导的心脏的射血分数及短轴缩短率。生化检测显示，与对照组相比，LPS组SOD血清水平下降，MDA、CK-MB及LDH血清水平升高，EA可呈剂量依赖性上调SOD，下调MDA、CK-MB及LDH的血清水平。蛋白免疫印迹显示EA预处理可上调核转录因子红系2相关因子2（Nrf2）以及血红素加氧酶1（HO-1）的水平。结论 EA可有效改善LPS诱导的心功能不全，可能是通过减轻氧化应激水平及上调Nrf2/HO-1水平发挥作用。
Abstract:
Objective To investigate the probable mechanism of ellagic acid(EA)’s preventive effect on cardiac insufficiency caused by lipopolysaccharide (LPS)-induced sepsis. Methods 32 C57BL/6 mice were placed into four groups: C ontrol(Con, n=8), LPS( n=8), 20EA( n=8), and 40EA( n=8). 20EA and 40EA groups were administered EA for 14 d,followed by intraperitoneal injections of 10 mg/kg LPS and saline. The heart function was evaluated using echocardiography,ejection fraction and short fractional shortening were assessed,and serum levels of superoxide dismutase(SOD),malondialdehyde(MDA),creatine kinase isoenzyme(CK-MB),and lactate dehydrogenase(LDH) were tested using a biochemical approach. Results Echocardiography showed that EA improved LPS-induced cardiac ejection fraction and short-axis shortening in a dose-dependent manner. Biochemical assays showed that compared with the control group , levels of SOD decreased and levels of MDA,CK-MB and LDH in the serum increased in the LPS group. EA improved SOD and decreased the serum levels of MDA,CK-MB and LDH in a dose-dependent way. The western blot results indicate that EA pretreatment could increase nuclear factor erythroid 2-related factor 2(Nrf2) and hemeoxygenase-1(HO-1) levels. Conclusion EA can significantly ameliorate the cardiac dysfunction generated by LPS ,which may play a role in reducing the level of oxidative stress and elevating the level of Nrf2/HO-1

参考文献/References:

[1] Dickson K,Lehmann C. Inflammatory response to different toxins in experimental sepsis models[J]. Int J Mol Sci,2019,20(18):4341.

[2] Tan Y,Wan H,Sun M,et al. Cardamonin protects against lipopolysaccharide-induced myocardial contractile dysfunction in mice through Nrf2-regulated mechanism[J]. Acta Pharmacol Sin,2021,42(3):404-413.

[3] Wei A,Liu J,Li D,et al. Syringaresinol attenuates sepsis-induced cardiac dysfunction by inhibiting inflammation and pyroptosis in mice[J]. Eur J Pharmacol,2021,913:174644.

[4] Priyadarsini KI,Khopde SM,Kumar SS,et al. Free radical studies of ellagic acid,a natural phenolic antioxidant[J]. J Agric Food Chem,2002,50(7):2200-2206.

[5] Yu YM,Chang WC,Wu CH,et al. Reduction of oxidative stress and apoptosis in hyperlipidemic rabbits by ellagic acid[J]. J Nutr Biochem,2005,16(11):675-681.

[6] Lin MC,Yin MC. Preventive effects of ellagic acid against doxorubicin-induced cardio-toxicity in mice[J]. Cardiovasc Toxicol,2013,13(3):185-193.

[7] Aishwarya V,Solaipriya S,Sivaramakrishnan V. Role of ellagic acid for the prevention and treatment of liver diseases[J]. Phytother Res,2021,35(6):2925-2944.

[8] Gupta A,Singh AK,Kumar R,et al. Neuroprotective potential of ellagic acid: a critical review[J]. Adv Nutr,2021,12(4):1211-1238.

[9] Liu YC,Yu MM,Shou ST,et al. Sepsis-induced cardiomyopathy: mechanisms and treatments[J]. Front Immunol,2017,8:1021.

[10] Pathan N,Franklin JL,Eleftherohorinou H,et al. Myocardial depressant effects of interleukin 6 in meningococcal sepsis are regulated by p38 mitogen-activated protein kinase[J]. Crit Care Med,2011,39(7):1692-1711.

[11] Noori M,Jafari B,Hekmatdoost A. Pomegranate juice prevents development of non-alcoholic fatty liver disease in rats by attenuating oxidative stress and inflammation[J]. J Sci Food Agric,2017,97(8):2327-2332.

[12] Jones DP. Radical-free biology of oxidative stress[J]. Am J Physiol Cell Physiol,2008,295(4):C849-C868.

[13] Gu J,Luo L,Wang Q,et al. Maresin 1 attenuates mitochondrial dysfunction through the ALX/cAMP/ROS pathway in the cecal ligation and puncture mouse model and sepsis patients[J]. Lab Invest,2018,98(6):715-733.

[14] Li D,Wang M,Ye J,et al. Maresin 1 alleviates the inflammatory response,reduces oxidative stress and protects against cardiac injury in LPS-induced mice[J]. Life Sci,2021,277:119467.

[15] Pari L,Sivasankari R. Effect of ellagic acid on cyclosporine A-induced oxidative damage in the liver of rats[J]. Fundam Clin Pharmacol,2008,22(4):395-401.

[16] Feng J,Liu Z,Chen H,et al. Protective effect of cynaroside on sepsis-induced multiple organ injury through Nrf2/HO-1-dependent macrophage polarization[J]. Eur J Pharmacol,2021,911:174522.

[17] ALTamimi JZ,AlFaris NA,Aljabryn DH,et al. Ellagic acid improved diabetes mellitus-induced testicular damage and sperm abnormalities by activation of Nrf2[J]. Saudi J Biol Sci,2021,28(8):4300-4310.

[18] Xiao Y,Huang R,Wang N,et al. Ellagic acid alleviates oxidative stress by mediating Nrf2 signaling pathways and protects against paraquat-induced intestinal injury in piglets[J]. Antioxidants (Basel),2022,11(2):252.

[19] Alfaris NA,Alshammari GM,Altamimi JZ,et al. Ellagic acid prevents streptozotocin-induced hippocampal damage and memory loss in rats by stimulating Nrf2 and nuclear factor-kappaB,and activating insulin receptor substrate/PI3K/Akt axis[J]. J Physiol Pharmacol,2021,72(4):503-515.

[20] Wei YZ,Zhu GF,Zheng CQ,et al. Ellagic acid protects dopamine neurons from rotenone-induced neurotoxicity via activation of Nrf2 signalling[J]. J Cell Mol Med,2020,24(16):9446-9456.

[21] Gu L,Deng WS,Liu Y,et al. Ellagic acid protects Lipopolysaccharide/D-galactosamine-induced acute hepatic injury in mice[J]. Int Immunopharmacol,2014,22(2):341-345.

[22] Yüce A,Ate??ahin A,?eriba?? AO,et al. Ellagic acid prevents cisplatin-induced oxidative stress in liver and heart tissue of rats[J]. Basic Clin Pharmacol Toxicol,2007,101(5):345-349.

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更新日期/Last Update: 2023-03-23