[1]许骁 李泽衍 周雨扬 聂鹂庆 江洪.下丘脑腹内侧核激活对大鼠急性心肌梗死后心脏电活动的影响[J].心血管病学进展,2022,(4):375-379.[doi:10.16806/j.cnki.issn.1004-3934.20.04.021]
　(Department of Cardiology,Renmin Hospital of Wuhan University Cardiac Autonomic Nerve Research Center of Wuhan University,Cardiovascular Research Institute of Wuhan University,et al.The Effect of Ventromedial Hypothalamic Nucleus Activation on Cardiac Electrical Activity after Acute Myocardial Infarction in Rats[J].Advances in Cardiovascular Diseases,2022,(4):375-379.[doi:10.16806/j.cnki.issn.1004-3934.20.04.021]
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下丘脑腹内侧核激活对大鼠急性心肌梗死后心脏电活动的影响()
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《心血管病学进展》[ISSN:51-1187/R/CN:1004-3934]

卷:
期数:
2022年4期
页码:
375-379
栏目:
论著
出版日期:
2022-04-25

文章信息/Info

Title:
The Effect of Ventromedial Hypothalamic Nucleus Activation on Cardiac Electrical Activity after Acute Myocardial Infarction in Rats
作者:
许骁 李泽衍 周雨扬 聂鹂庆 江洪
(武汉大学人民医院心内科 武汉大学心脏自主神经研究中心 武汉大学心血管病研究所 心血管病湖北省重点实验室,湖北 武汉 430060)
Author(s):
(Department of Cardiology;  Renmin Hospital of Wuhan University Cardiac Autonomic Nerve Research Center of Wuhan University;  Cardiovascular Research Institute of Wuhan University;  Hubei Key Laboratory of Cardiology;  Wuhan 430060;  Hubei;  China)
关键词:
下丘脑腹内侧核; 化学遗传; 心肌梗死; 心电活动
Keywords:
Ventromedial hypothalamic nucleus;  Chemogenetic;  Myocardial infarction;  Cardiac electrical activity
DOI:
10.16806/j.cnki.issn.1004-3934.20.04.021
摘要:
目的 探究激活下丘脑腹内侧（VMH）对大鼠急性心肌梗死AMI）后心脏电活动的影响。方法 将15只大鼠随机分为对照组、组和激活组，每组5只。激活组在VMH区域注射化学遗传激活病毒rAAV-hSyn-hM3d(Gq)-EGFP-WPRE-pA2/2，组在VMH区域注射空载病毒rAAV-hSyn-EGFP-WPRE-pA2/2。病毒注射3周后，各组接受氯氮平-N-氧化物腹腔注射2周。病毒注射5周后建立模。对照组大鼠接受假手术处理。造模24 h后，测量各组大鼠心电图以左心室有效不应期（ERP）。取大鼠脑组织冰冻切片做免疫荧光染色，观察大鼠VMH区域荧光蛋白表达情况，测量各组大鼠VMH c-fos表达量。结果 （1）VMH c-fos表达量均显著于对照组，且激活组VMH c-fos表达量显著于组[激活组vs组：25.395.16 vs 18.76±3.52，P0.05]。（2）对照组相比，组激活组心率变异性频域分析指标频功率（LF）升，频功率（HF）降，LF/HF比值升；组相比，激活组LF升，HF降，LF/HF比值升。上述结果均有显著统计学差异（P0.01）（3）对照组相比，组PR间期、QRS波时限升，但结果无显著性差异；组QT间期显著延（P0.01）。组相比，激活组PR间期、QRS波时限QT间期均显著延（P0.05）（4）在体电生理实验结果显示，左心室心尖部、心中部心底部ERP均显著降，且激活组ERP显著于组（P0.05）。结论 激活VMH可加重大鼠后心脏电活动紊乱。
Abstract:
Objective To explore the effect of ventromedial hypothalamus nucleus (VMH) activation on cardiac electrical activity after acute myocardial infarction (AMI) in rats. Methods A total of 15 rats were randomly divided into controlmyocardial infarction (MI) and activated groups, with 5 in each group. The activated group was injected with chemical genetic virus rAAV-hSyn-hM3d(Gq)-EGFP-WPRE-pA2/2 in VMH region,and the MI group injected with empty virus rAAV-hSyn-EGFP-WPRE-pA2/2. 3 weeks after virus injection,all rats received intraperitoneal injection of clozapine-N-oxide for 2 weeks. AMI model was established and the rats in the control group received sham operation. 24 hours later,ECG and left ventricular effective refractory period (ERP) were recorded. Brains were frozen-sectioned for immunofluorescence staining. Expressions of fluorescent proteins and c-fos were measured. Results (1)The expressions of c-fos were significantly higher in MI and activated groups than control,and higher in activated group than MI (activated vs MI:25.39±5.16 vs 18.76±3.52,P＜0.05). (2)Compared with control group,low frequency power (LF) of heart rate variability index was increased in MI and activated groups,and high frequency power (HF) decreased,the ratio of LF/HF increased. Compared with MI group,LF was increased in activated group,and HF decreased,the ratio of LF/HF increased. There were significant differences on above results (P＜0.01). (3)Compared with control group,PR interval and QRS wave duration were increased in MI group,yet with no significant difference (P＞0.05),and QT interval prolonged (P＜0.01). Compared with MI group,PR interval,QRS wave duration and QT interval were prolonged in activated group (P＜0.05). (4)The results of electrophysiological experiments in vivo showed that ERP of the left ventricular apex (LVA), the left ventricular base (LVB), and the median area between the LVA and the LVB in MI group and activation group were significantly shorter and ERP were obviously shorter in activated groups than MI (P＜0.05). Conclusion The activation of VMH may aggravate the disturbance of cardiac electrical activity after AMI in rats.

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备注/Memo

备注/Memo:
收稿日期：2021-08-05
基金项目：国家自然科学基金(81970287)
更新日期/Last Update: 2022-05-13