[1]阮怀玉 廖小婷 曾彬.miR-19b对H2O2诱导的大鼠心肌细胞H9c2氧化应激损伤的保护作用及机制研究[J].心血管病学进展,2021,(2):177.[doi:10.16806/j.cnki.issn.1004-3934.2021.02.020]
　RUAN Huaiyu,LIAO Xiaoting,ZENG Bin.Protective Effects and Mechanisms of miR-19b on the Oxidative Stress Injury induced by Hydrogen Peroxide in H9c2 Myocardial Cells[J].Advances in Cardiovascular Diseases,2021,(2):177.[doi:10.16806/j.cnki.issn.1004-3934.2021.02.020]
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miR-19b对H2O2诱导的大鼠心肌细胞H9c2氧化应激损伤的保护作用及机制研究()
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《心血管病学进展》[ISSN:51-1187/R/CN:1004-3934]

卷:
期数:
2021年2期
页码:
177
栏目:
论著
出版日期:
2021-02-25

文章信息/Info

Title:
Protective Effects and Mechanisms of miR-19b on the Oxidative Stress Injury induced by Hydrogen Peroxide in H9c2 Myocardial Cells
作者:
阮怀玉 廖小婷 曾彬
 (武汉大学人民医院心内科 武汉大学心血管病研究所 心血管病湖北省重点实验室,湖北 武汉 430060 )
Author(s):
RUAN Huaiyu; LIAO Xiaoting; ZENG Bin
( Department of CardiologyRenmin Hospital of Wuhan UniversityCardiovascular Disease Institute of Wuhan UniversityHubei Key Laboratory of Cardiovascular DiseasesWuhan 430060 HubeiChina)
关键词:
miR-19b; H9c2心肌细胞; 氧化应激损伤; 凋亡; Nrf2/ HO-1信号通路
Keywords:
miR-19b;  H9c2 mycocardial cell;  Oxidative stress injury;  Apoptosis; Nrf2/HO-1signal pathway
DOI:
10.16806/j.cnki.issn.1004-3934.2021.02.020
摘要:
目的 研究 microRNA(miR)-19b是否在H2O2诱导的心肌细胞的氧化应激损伤中具有保护作用。方法 将大鼠H9c2心肌细胞株分为6组，空白对照组(L1)，H2O2组(L2)，H2O2+miR-19b mimic阴性对照(NC)组(L3)，H2O2+miR-19b mimic组(L4)，H2O2+miR-19b inhibitor组(L5)， H2O2+miR-19b inhibitor NC组(L6)。NC组细胞转染随机合成的miRNA片段作为阴性对照。采用CCK8和流式细胞术检测细胞活性和凋亡情况，逆转录聚合酶链式反应（RT-PCR）法检测miR-19b的表达量，并利用试剂盒检测超氧化物歧化酶（SOD）、乳酸脱氢酶（LDH）、丙二醛（MDA）和还原型谷胱甘肽（GSH）水平，Western blot法检测核因子相关因子2（Nrf2）、血红素氧合酶1（HO-1）表达水平。结果 与L1组比较，L2组和L3组中miR-19b表达量、H9c2细胞活性和细胞内SOD、GSH水平均显著降低（P0.01），细胞凋亡率和细胞内LDH、MDA水平、Nrf2和HO-1表达水平均显著增加（P0.01）。与L2组及L3组比较，L4组中miR-19b表达量、H9c2细胞活性和细胞内SOD、GSH水平、Nrf2和HO-1表达水平均显著增加（P0.01），细胞凋亡率和细胞内LDH、MDA水平均显著降低（P0.01），L5组中miR-19b表达量和H9c2细胞活性和细胞内SOD、GSH水平、Nrf2和HO-1表达水平均显著降低（P0.01），细胞凋亡率和细胞内LDH、MDA水平均显著增加（P0.01），L6组miR-19b表达量、H9c2细胞活性、细胞凋亡率、细胞内SOD、GSH、LDH、MDA水平以及Nrf2和HO-1表达水平均未见统计学差异（P0.05）。结论 miR-19b 高表达可显著抑制H9c2细胞的氧化应激损伤，发挥心肌细胞保护作用。
Abstract:
Objective To investigate whether microRNA-19b (miR-19b) has a protective effect on H2O2-induced oxidative stress injury in rat myocardial cells. Methods Rat H9c2 myocardial cells were divided into 6 groups as follows:blank control(L1),H2O2(L2),H2O2+miR-19b mimic negative control(NC)(L3),H2O2+miR-19b mimic(L4),H2O2+miR-19b inhibitor(L5) and H2O2+miR-19b inhibitor NC(L6). Cells transfected with random miRNA fragments serve as NC. Cell activity and apoptosis were analyzed by CCK8 and flow cytometry. The expression of miR-19b was detected by RT-PCR. Superoxide dismutase(SOD),lactic dehydrogenase(LDH),malondialdehyde(MDA),glutathione(GSH) levels were tested by related kits and the expression of Nrf2 and HO-1 were decected by Westernblot. Results Compared with L1 group,the expression of miR-19b,cell viability,SOD and GSH levels were significantly decreased(P0.01) and cell apoptotic rate,LDH and MDA levels,Nrf2 and HO-1 proteins increased (P0.01)in L2 and L3 group. Compared with L2 and L3 group,the expression of miR-19b,SOD and GSH levels,Nrf2 and HO-1 proteins were markedly elevated(P0.01) and cell apoptotic rate,LDH and MDA levels dropped(P0.01) in L4 group. Meanwhile,the expression of miR-19b,cell viability,SOD and GSH levels,Nrf2 and HO-1 proteins were markedly declined(P0.01) and cell apoptotic rate,LDH and MDA levels elevated(P0.01) in L5 group,yet all of which showed no significant differences in L6 group(P0.05). Conclusion MiR-19b could inhibits oxidative stress damage in H9c2 cells and play a protective role on myocardial cells.

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更新日期/Last Update: 2021-06-16