[1]李强 陆士娟 黄康 张伟 钟赞蕊.MiR-181a和MiR-216b在冠心病患者外周血单个核细胞中表达及意义[J].心血管病学进展,2019,(8):1187-1192.[doi:10.16806/j.cnki.issn.1004-3934.2019.08.029]
　LI Qiang,LU Shijuan,HUANG Kang,et al.Expression and Significance of miR-181a and miR-216b in Peripheral Blood Mononuclear Cells of Patients with Coronary Heart Disease[J].Advances in Cardiovascular Diseases,2019,(8):1187-1192.[doi:10.16806/j.cnki.issn.1004-3934.2019.08.029]
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MiR-181a和MiR-216b在冠心病患者外周血单个核细胞中表达及意义()
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《心血管病学进展》[ISSN:51-1187/R/CN:1004-3934]

卷:
期数:
2019年8期
页码:
1187-1192
栏目:
论著
出版日期:
2019-11-25

文章信息/Info

Title:
Expression and Significance of miR-181a and miR-216b in Peripheral Blood Mononuclear Cells of Patients with Coronary Heart Disease
作者:
李强 陆士娟 黄康 张伟 钟赞蕊
(中南大学湘雅医学院附属海口医院心血管内科，海南 海口 570206)
Author(s):
LI Qiang;  LU Shijuan;  HUANG Kang;  ZHANG Wei;  ZHONG Zanrui
?(Department of Vasculocardiology, Haikou Hospital Affiliated to Xiangya Medicine School, Central South University,Haikou 570206,Hainan,China)
关键词:
miR-181a; miR-216b; 冠心病; 外周血单个核细胞
Keywords:
MiR-181a;  MiR-216b;  Coronary heart disease;  Peripheral blood mononuclear cell
DOI:
10.16806/j.cnki.issn.1004-3934.2019.08.029
摘要:
目的探究miR-181a和miR-216b在冠心病（CHD）患者外周血单个核细胞（PBMC）中表达及意义。 方法实时定量PCR检测125例CHD患者（观察组）50例体检健康者（对照组）PBMC中miR-181amiR-216b的表达水平。向CHD患者PBMC中分别转染miR-181a（miR-181a组）、miR-216b（miR-216b组）对照质粒（NC组）。利用实时定量PCR检测各组细胞miR-181amiR-216b的表达水平，采用MTT法检测各组细胞增殖、流式细胞术检测细胞凋亡、划痕实验检测细胞迁移。 结果观察组患者PBMC中miR-181amiR-216b的表达水平均明显低于对照组（P<0.05）。转染后，miR-181a组PBMC中miR-181a表达水平miR-216b组miR-216b表达水平均分别高于NC组（P<0.05）。随着孵育时间延长，miR-181a组miR-216b组细胞增殖率无明显变化凋亡率明显增加（P<0.05）；NC组细胞增殖率明显增加（P<0.05），凋亡率无明显变化。孵育相同时间时，miR-181a组miR-216b组细胞增殖率划痕愈合率明显低于NC组（P<0.05），细胞凋亡率明显高于NC组（P<0.05）。结论miR-181a和miR-216b在CHD患者PBMC中低表达，提升miR-181amiR-216b的表达量可明显抑制PBMC增殖迁移，诱导其发生凋亡。
Abstract:
Objective To explore expression and significance of miR-181a and miR-216b in peripheral blood mononuclear cells(PBMC) of patients with coronary heart disease (CHD). Methods Real-time quantitative PCR was performed to detect expression levels of miR-181a and miR-216b in PBMC of 125 CHD patients (observation group) and 50 healthy people who underwent physical examination(control group). MiR-181a(miR-181a group), miR-216b (miR-216b group) and control plasmid (NC group) were transfected into PBMC of CHD patient respectively. Real-time quantitative PCR was performed to detect expression levels of miR-181a and miR-216b in each group. Cell proliferation was detected by MTT assay, apoptosis was detected by flow cytometry. And cell migration was detected by scratch assay. Results The expression levels of miR-181a and miR-216b in PBMC of observation group were significantly lower than those of control group (P<0.05). After transfection, expression levels of miR-181a in miR-181a group and miR-216b in miR-216b group were higher than those in NC group respectively (P<0.05). With prolongation of incubation time, there was no significant change in cell proliferation rate in miR-181a group and miR-216b group, while apoptosis rates were significantly increased (P<0.05). The cell proliferation rate in NC group was significantly increased (P<0.05), and there was no significant change in apoptosis rate. When incubation time was the same, cell proliferation rate and scratch healing rate in miR-181a group and miR-216b group were significantly lower than those in NC group (P<0.05), while apoptosis rate was significantly higher than that in NC group (P<0.05). Conclusion MiR-181a and miR-216b are lowly expressed in PBMC of CHD patients. Increasing expression quantities of miR-181a and miR-216b can significantly inhibit proliferation and migration of PBMC, and induce apoptosis.

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更新日期/Last Update: 2020-03-02