[1]柯伟良 李日健 闫海 冯锦山 邵芳芳 李兴岳.hTERT基因对移植的内皮祖细胞在体内的增殖和血管新生的影响[J].心血管病学进展,2019,(5):822-827.[doi:10.16806/j.cnki.issn.1004-3931.2019.05.039]
　KE Weiliang,LI Rijian,YAN Hai,et al.Effect of hTERT Gene on Proliferation and Angiogenesis of Transplanted Endothelial Progenitor Cells in Vivo[J].Advances in Cardiovascular Diseases,2019,(5):822-827.[doi:10.16806/j.cnki.issn.1004-3931.2019.05.039]
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hTERT基因对移植的内皮祖细胞在体内的增殖和血管新生的影响()
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《心血管病学进展》[ISSN:51-1187/R/CN:1004-3934]

卷:
期数:
2019年5期
页码:
822-827
栏目:
论著
出版日期:
2019-08-25

文章信息/Info

Title:
Effect of hTERT Gene on Proliferation and Angiogenesis of Transplanted Endothelial Progenitor Cells in Vivo
作者:
柯伟良 李日健 闫海 冯锦山 邵芳芳 李兴岳
Author(s):
KE Weiliang;  LI Rijian;  YAN Hai;  FENG Jinshan;  SHAO Fangfang; LI Xingyue; LI Shanghai
(Department of Cardiology,The Affiliated Hospital of Guangdong Medical University,Guangzhou 524001,Guangdong,China)
关键词:
hTERT; 内皮祖细胞; 增殖; 血管新生
Keywords:
hTERT;  ndothelial progenitor cells;  Proliferation;  Migration;  Angiogenesis
DOI:
10.16806/j.cnki.issn.1004-3931.2019.05.039
摘要:
目的研究基因对移植的内皮祖细胞在体内的增殖和血管新生影响的机制。方法EPC转染和-EPC转染选择三个梗病灶区域通过心肌内注射移植EPC和-EPCs，对照组的大鼠注射PBS）；通过苏木精-曙红染色的心肌的石蜡切片观察心肌梗区域的毛细血管再生通过RT-qPCR检测血管内皮细胞标记物（CD31、CD34、CD105和CD146）mRNA的表达量使用酶标仪NO浓度乳酸脱氢酶LDH）浓度测定试剂盒iNOS活性水平通过酶联免疫吸附试验试剂盒检测VEGF、VEGFR和MAPK蛋白含量结果 与EPC转染组相比，hTERT-EPC转染组毛细血管在心肌梗区增生较多EPC转染组CD31、CD34、CD105CD146 mRNA和蛋白表达水平增加，差异有统计学意义（P<0.05）差异有统计学意义（P<0.05）中NO18.23±3.15）、LDH（4.03±0.44）和iNOS4.52±1.13）的分泌均高于EPC转染组14.62±2.18）、（3.24±0.67）、（3.83±0.82）]，差异有统计学意义（P<0.05）hTERT-EPC转染组VEGF、VEGFR和MAPK与EPC转染组相比升高，差异有统计学意义（P<0.05）结论hTERT基因移植的内皮祖细胞在体内的增殖和血管新生
Abstract:
Objective he mechanism of hTERT gene on the proliferation and angiogenesis of transplanted endothelial progenitor cell(EPC) in vivo. Methods Ninety healthy male Sprague-Dawley rats(200～300 g) were randomly divided into three groups:control group(n=30),EPC transfection group(n=30) and hTERT-EPC transfection group(n=30). Three infarct lesion areas were selected. Transplanted EPC and hTERT-EPC were injected into myocardium, and PBS was injected into rats in the control group. The capillary regeneration of myocardial infarction area was observed by paraffin section of myocardium stained with hematoxylin-eosin. The mRNA and protein expression levels of vascular endothelial cell markers(CD31, CD34, CD105 and CD146) were detected by RT-qPCR and western blotting. The effect of hTERT on EPC function was assessed by detecting the proliferation of bone marrow EPCs in vitro in mice of control group and model group. NO concentration and LDH concentration were detected by the enzyme labelling instrument, and the iNOS activity level was detected by commercial assay kits. VEGF, VEGFR and MAPK protein contents were detected by ELISA Kit. Results Compared with EPC transfection group, capillaries in hTERT-EPC transfection group proliferated was more in myocardial infarction group; compared with EPC transfection group, the expression levels of CD31, CD34, CD105 and CD146 mRNA and protein in hTERT-EPC transfection group increased, the differences were statistically significant(P<0.05); compared with EPC of the control group (173.52±20.17), the model group (32.14±12.33) showed impaired proliferation and migration, hTERT-EPC group (122.14±13.44) increased the proliferation and migration of damaged EPC, the difference was statistically significant(P<0.05); in the hTERT-EPC transfection group, the secretion of NO (18.23±3.15), LDH (4.03±0.44) and iNOS (4.52±1.13) was higher than those of EPC transfection group [(14.62±2.18), (3.24±0.67), (3.83±0.82)], the differences were statistically significant(P<0.05); the levels of VEGF,VEGFR and MAPK hTERT-EPC transfection group were significantly higher than those of EPC transfection group, the differences were statistically significant(P<0.05). Conclusion The hTERT gene promotes the proliferation and angiogenesis of transplanted endothelial progenitor cells in vivo

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备注/Memo

备注/Memo:
收稿日期：2019-04-12
更新日期/Last Update: 2019-12-25